Skip to content

Microbial production of commercial enzymes (Amylase and pectinase) from agrowaste

By Praveen Kumar Gupta, Gayatri R, Lavanya S

Department of Biotechnology, R V College of Engineering, Bangalore, Karnataka, India.

About 81.4 million tons of agricultural waste (AW) and 50 million tons of Fruit and vegetable waste (FVW) are discarded every year in India, which has a worth of US$ 483.9 million. Because of the size of FVW and AW generated, transformation of such waste to commercial products like enzymes (pectinase, amylase) becomes essential (Fig.1). Polysaccharide components (like cellulose, pectin and starch) present in FVW and AW can be utilized by means of microbial bio-processing for the production of commercial enzymes.

MARKET SIZE FOR AMYLASE AND PECTINASE :

The market size for alpha amylase in the year 2018 is 278.23 million $ and it is estimated to reach 352.78 million $ by the year 2026 at a CAGR (Compound Annual Growth Rate). The market size for pectinase in the year 2019 is 30 million $ and it is estimated to reach 35.5 million $ by the year 2024 (Global Pectinase Market Research Report, 2019).

  • The Story Of Emily Rose : Was it epilepsy or the six serpents?
    Anneliese Michel, a collateral damage in the never ending ideological battle between science and religion. Anneliese is an example for what would happen if we leave the work of doctors to priests.
  • Exploration on Ants and their social benefits for surviving the world
    An ant colony refers to the social rules ants follow to organise themselves and work. Each colony contains thousands of ants and are located underground most of the time. In terms of an ant society, there are four different types of ants. A colony can be termed as a superorganism with multiple members each having a role of their own to play to make the colony survive as a single unit.

UTILIZATION OF AGRO-WASTE FOR THE PRODUCTION OF AMYLASE AND PECTINASE :

Microbial production of amylase requires starch as a substrate. Cassava bagassecontains50% starch content. Other agro-wastes containing starch content are: corn bran (11.2%); corn fiber (15-20%);potato (67.5%); pineapple stem (11%);mango kernel (58%);jackfruit seeds (60-80%);avocado (27.5-29.8%) and banana peel(30%). Similarly, microbial production of pectinase requires pectin as a substrate. Agro-wastes containing pectin are: mango peel (10-15%), banana peel (4-6%) and apple pomace (18-19%). Submerged fermentation (SmF) and solid-state fermentation (SSF) are the two immensely used fermentation processes for the production of amylase and pectinase enzyme. SmF and SSF are applicable for bacteria, yeast and fungal species. The microbes reported for the production of amylase utilizing ago-waste are Aspergillus oryzae, Streptomyces sp., Bacillus subtilis, Rhizopus oryzae, Penicillium expansum. The microbes reported for the production of pectinase utilizing agro-waste are Erwinia sp., Streptomyces, Lactobacillus, Aspergillus niger, A. oryzae, Penicillium, Fusarium, Mucor, Neurospora crassa and Saccharomyces sp.

APPLICATIONS OF AMYLASE AND PECTINASE :

Amylases are involved in various applications like starch coating, deinking, biopolishing, desizing, modification of synthetic fibers, stain cleaning etc. Pectinases are involved in various applications like fruit juice clarification, coffee and tea fermentation, waste water treatment, bio-scouring of plant fibres etc.

CONCLUSION :

Agro-waste can provide cheaper substrate platform for the production of commercial enzymes like amylase and pectinase. Utilization of agro waste can prevent breeding of disease-causing microbes and can also reduce soil and water pollution.

3 Comments »

  1. Very informative . The information provided here is very much required in the present day scenario for sustainable environment

    Like

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google photo

You are commenting using your Google account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s

%d bloggers like this: